Anticorps Polyclonal de lapin anti-PES1

• Phare
• Validé par KD/KO

PES1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13553-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of COLO 320 using 13553-1-AP (same clone as 13553-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 13553-1-AP (same clone as 13553-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  IP experiment of COLO 320 using 13553-1-AP (same clone as 13553-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:300. This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 13553-1-AP (same clone as 13553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 13553-1-AP (same clone as 13553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 13553-1-AP (same clone as 13553-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13553-1-AP (PES1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

13553-1-PBS cible PES1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PES1 Protéine recombinante Ag4342
• Nom complet: pescadillo homolog 1, containing BRCT domain (zebrafish)
• Masse moléculaire calculée: 588 aa, 68 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC032489
• Symbole du gène: PES1
• Identification du gène (NCBI): 23481
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}