Anticorps Polyclonal de lapin anti-PEX1

• Phare
• Validé par KD/KO

PEX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 13669-1-AP

Synonymes

EC:3.6.4.-, Peroxin 1, Peroxin-1, Peroxisomal ATPase PEX1, Peroxisome biogenesis factor 1

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Galerie de données de validation

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  WB analysis of Jurkat using 13669-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13669-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 13669-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 13669-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13669-1-AP

  Immunofluorescent analysis of HeLa cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

13669-1-AP cible PEX1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PEX1 Protéine recombinante Ag4623
• Nom complet: peroxisomal biogenesis factor 1
• Masse moléculaire calculée: 1283 aa, 143 kDa
• Poids moléculaire observé: 143 kDa
• Numéro d’acquisition GenBank: BC035575
• Symbole du gène: PEX1
• Identification du gène (NCBI): 5189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxin (PEX) proteins generate and maintain peroxisomes. The peroxin-1 (PEX1) ATPase facilitates the recycling of the peroxisome matrix protein receptor PEX5 and is the most commonly affected peroxin in human peroxisome biogenesis disorders (PMID: 28600347).  PEX1 and PEX6 are the only members of the AAA family (for ATPases associated with diverse cellular) activities implicated in peroxisome biogenesis and are closely related to p97, which functions in endoplasmic reticulum-associated protein degradation to retrotranslocate endoplasmic reticulum proteins to the cytosol (PMID: 9695811; 11740563).

Publications

Species	Application	Title
	IF	Autophagy; Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.; Authors - Marcus Nordgren
human	WB,IF	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
human	WB	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni
mouse	WB	Cell Commun Signal; TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions; Authors - Laxman Manandhar
human	IHC	Eur J Hum Genet; Spectrum of PEX1 and PEX6 variants in Heimler syndrome.; Authors - Claire E L Smith
human	WB	Mol Ther Methods Clin Dev; AAV-mediated PEX1 gene augmentation improves visual function in the PEX1-Gly844Asp mouse model for mild Zellweger spectrum disorder.; Authors - Catherine Argyriou

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