Anticorps Polyclonal de lapin anti-PEX12

PEX12 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 27011-1-AP

Synonymes

PEX, Peroxisome assembly protein 12, Peroxisome assembly factor 3, Peroxin-12, Peroxin 12

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Galerie de données de validation

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  WB analysis of MCF-7 using 27011-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 27011-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 27011-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27011-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules HeLa, cellules HepG2, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

27011-1-AP cible PEX12 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PEX12 Protéine recombinante Ag25500
• Nom complet: peroxisomal biogenesis factor 12
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 40-42 kDa
• Numéro d’acquisition GenBank: BC031085
• Symbole du gène: PEX12
• Identification du gène (NCBI): 5193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxisome assembly protein 12 (PEX12), also known as Peroxisome assembly factor 3 (PAF-3), is an integral peroxisomal membrane protein that is essential for peroxisomal matrix protein import and is one of only two peroxins known to interact with the ligand-binding domain of PEX5. Mutations in human PEX12 result in Zellweger syndrome, a lethal neurological disorder, and implicate the zinc ring domain in PEX12 function (PMID: 12456682). Pex12 exhibits protein-Ub ligase activity with specificity for the E2 protein Pex4 and Ubc4 (PMID: 19687296).

Publications

Species	Application	Title
mouse	WB	Commun Biol; PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3; Authors - Jia-Teng Sun
pig	WB	PLoS Pathog; SIRT5-mediated desuccinylation of the porcine deltacoronavirus M protein drives pexophagy to enhance viral proliferation; Authors - Zhuang Li

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