Anticorps Polyclonal de lapin anti-PEX3

• Phare
• Validé par KD/KO

PEX3 Polyclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 10946-1-AP

Synonymes

Peroxin 3, Peroxin-3, PEX

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Galerie de données de validation

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  WB analysis of HeLa using 10946-1-AP

  WB result of PEX3 antibody (10946-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX3 transfected HeLa cells.

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  IF Staining of mouse brain using 10946-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PEX3 antibody (10946-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 10946-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PEX3 antibody (10946-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IF-P: tissu rénal de souris, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 1 publications below

Informations sur le produit

10946-1-AP cible PEX3 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, arabidopsis
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PEX3 Protéine recombinante Ag1384
• Nom complet: peroxisomal biogenesis factor 3
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC014551
• Symbole du gène: PEX3
• Identification du gène (NCBI): 8504
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 3, also known as PEX3 (peroxisomal biogenesis factor 3) or TRG18, is a 373 amino acid multi-pass membrane protein that anchored in the peroxisomal membrane and essential for peroxisome biogenesis and integrity. Additionally, Peroxin 3 acts as a docking factor for Peroxin 19 and is required for the import of peroxisomal proteins. Defects in the gene encoding Peroxin 3 are the cause of peroxisome biogenesis disorder complementation group 12 (PBD-CG12) and Zellweger syndrome (ZwS), both of which arise from a failure of peroxisomal protein import.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.; Authors - Katharina M Walter
human	WB	Cell Death Differ; Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.; Authors - Weiwei Cui
mouse	WB	Kidney360; Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism.; Authors - Takeshi Terabayashi
mouse	WB	Methods Mol Biol; Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.; Authors - Miriam J Schönenberger
mouse	WB	Biochim Biophys Acta; Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.; Authors - Kovacs Werner J WJ
Arabidopsis	WB	J Integr Plant Biol; PEX16 contributions to peroxisome import and metabolism revealed by viable Arabidopsis pex16 mutants.; Authors - Sarah E Burkhart

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (07-14-2022)

BSA block in TBS

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293

PEX3 Antibody Western Blot validation (1:500 dilution) in HEK293 (Cat no:10946-1-AP)

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