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Anticorps Monoclonal anti-PEX5

PEX5 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C2C3

N° de cat : 66309-1-Ig

Synonymes

Peroxin 5, Peroxisome Marker, Peroxisome receptor 1, PEX5, PTS1 BP, PTS1 receptor, PTS1R, PXR1

Galerie de données de validation

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  • Western Blot (WB) analysis of HEK-293 cells using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of HEK-293 using 66309-1-Ig

    WB result of PEX5 antibody (66309-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX5 transfected HEK-293 cells.

  • Western Blot (WB) analysis of pig brain tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of pig brain using 66309-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of fetal human brain using 66309-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human liver using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human liver using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human kidney using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human kidney using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens).

Applications testées

Résultats positifs en WBtissu cérébral de porc, cellules HEK-293, tissu cérébral humain fœtal
Résultats positifs en IHCtissu hépatique humain, tissu rénal humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 1 publications below

Informations sur le produit

66309-1-Ig cible PEX5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

Réactivité Humain, porc, rat
Réactivité citéeHumain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène PEX5 Protéine recombinante Ag3268
Nom complet peroxisomal biogenesis factor 5
Masse moléculaire calculée 631 aa, 70 kDa
Poids moléculaire observé 70-80 kDa
Numéro d’acquisition GenBankBC010621
Symbole du gène PEX5
Identification du gène (NCBI) 5830
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder.

Protocole

Product Specific Protocols
WB protocol for PEX5 antibody 66309-1-IgDownload protocol
IHC protocol for PEX5 antibody 66309-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

BNIP3L/NIX regulates both mitophagy and pexophagy

Authors - Léa P Wilhelm
View Article