Anticorps Recombinant de lapin anti-CXCL4/PF4

CXCL4/PF4 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242741H5

N° de cat : 85738-1-PBS

Synonymes

C-X-C motif chemokine 4, CXCL4, PF-4, Platelet factor 4

---

Galerie de données de validation

• 

  WB analysis using 85738-1-RR (same clone as 85738-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85738-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85738-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood platelets using 85738-1-RR (same clone as 85738-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 85738-1-RR (PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85738-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85738-1-RR (same clone as 85738-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85738-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85738-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02069-1

  Cytometric bead array standard curve of MP02069-1, MOUSE CXCL4/PF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85738-1-PBS. Detection antibody: 85738-2-PBS. Standard: Eg3345. Range: 0.781-100 ng/mL.

---

Informations sur le produit

85738-1-PBS cible CXCL4/PF4 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CXCL4/PF4 Protéine recombinante Eg3345
• Nom complet: platelet factor 4
• Masse moléculaire calculée: 11kd
• Poids moléculaire observé: 10-14 kDa
• Numéro d’acquisition GenBank: NM_019932
• Symbole du gène: Pf4
• Identification du gène (NCBI): 56744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The chemokine (C-X-C motif) ligand 4 (CXCL4) is also named as PF4 (Platelet factor-4) and SCYB4. The platelets, the most abundant source of CXCL4 in vivo, control profibrotic macrophage activation via CXCL4. (PMID: 36807143). Exogenous CXCL4 drove profibrotic activation of monocyte-derived dendritic cells in vitro (PMID: 33042127). Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation.

{{ptg:RelatedPrimaryAntibodies}}