Anticorps Polyclonal de lapin anti-PFAS

• Phare
• Validé par KD/KO

PFAS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24716-1-PBS

Synonymes

EC:6.3.5.3, FGAM synthase, FGAMS, FGAR amidotransferase, FGARAT

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Galerie de données de validation

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  WB analysis using 24716-1-AP (same clone as 24716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24716-1-AP (PFAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 24716-1-AP (same clone as 24716-1-PBS)

  WB result of PFAS antibody (24716-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFAS transfected HeLa cells. This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 24716-1-AP (same clone as 24716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24716-1-AP (PFAS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 24716-1-AP (same clone as 24716-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PFAS antibody (24716-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24716-1-PBS cible PFAS dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PFAS Protéine recombinante Ag20162
• Nom complet: phosphoribosylformylglycinamidine synthase
• Masse moléculaire calculée: 1338 aa, 145 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC167158
• Symbole du gène: PFAS
• Identification du gène (NCBI): 5198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.

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