Anticorps Recombinant de lapin anti-PFKFB2

PFKFB2 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243136E5

N° de cat : 85639-6-PBS

Synonymes

6PF-2-K/Fru-2,6-P2ase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, Fructose-2,6-bisphosphatase

---

Galerie de données de validation

• 

  WB analysis using 85639-6-RR (same clone as 85639-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85639-6-RR (PFKFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85639-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85639-6-RR

  Biolayer interferometry (BLl) kinetic assays of 85639-6-RR against Human PFKFB2 were performed. The affinity constant is 2.74 nM.

---

Informations sur le produit

85639-6-PBS cible PFKFB2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PFKFB2 Protéine recombinante Ag32347
• Nom complet: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• Masse moléculaire calculée: 505 aa, 58 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC075076
• Symbole du gène: PFKFB2
• Identification du gène (NCBI): 5208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PFKFB2(6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase 2) is also named as PFK-2/FBPase-2, which is a bifunctional enzyme responsible for the synthesis and breakdown of Fru-2,6-P2 in the regulation of glycolysis(PMID:10095107). In the C-terminal section, it belongs to the phosphoglycerate mutase family. The enzyme is a dimer of identical subunits, each composed of 505 amino acids and containing three discrete domains(PMID: 2552438). It has 2 isoforms produced by alternative splicing.

{{ptg:RelatedPrimaryAntibodies}}