Anticorps Polyclonal de lapin anti-PFKFB3

• Phare
• Validé par KD/KO

PFKFB3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(146)

Avis (5)

N° de cat : 13763-1-AP

Synonymes

PFKFB3-Specific, 6PF-2-K/Fru-2,6-P2ase 3, 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme, 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3

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Galerie de données de validation

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  WB analysis using 13763-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13763-1-AP

  WB result of PFKFB3-Specific antibody (13763-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKFB3-Specific transfected HEK-293 cells.

• 

  WB analysis of mouse heart using 13763-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13763-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13763-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 13763-1-AP

  IP result of anti-PFKFB3 (IP:13763-1-AP, 4ug; Detection:13763-1-AP 1:500) with mouse spleen tissue lysate 2440 ug.

• 

  IP experiment of mouse spleen using 13763-1-AP

  IP result of anti-PFKFB3 (IP:13763-1-AP, 4ug; Detection:13763-1-AP 1:300) with mouse spleen tissue lysate 3920 ug.

• 

  IP experiment of mouse spleen using 13763-1-AP

  IP result of anti-PFKFB3 (IP:13763-1-AP, 4ug; Detection:13763-1-AP 1:300) with mouse spleen tissue lysate 3920 ug.

• 

  IHC staining of human stomach cancer using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13763-1-AP (PFKFB3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13763-1-AP (PFKFB3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13763-1-AP (PFKFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13763-1-AP (PFKFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13763-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PFKFB3-Specific antibody (13763-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HeLa, cellules Jurkat, tissu cardiaque de souris, tissu de thymus de souris, tissu splénique de souris
• Résultats positifs en IP: tissu splénique de souris,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 19 publications below
• WB: See 132 publications below
• IHC: See 31 publications below
• IF: See 21 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Informations sur le produit

13763-1-AP cible PFKFB3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PFKFB3 Protéine recombinante Ag4744
• Nom complet: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
• Masse moléculaire calculée: 520 aa, 60 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC040482
• Symbole du gène: PFKFB3
• Identification du gène (NCBI): 5209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. Its synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5.

Publications

Species	Application	Title
mouse	WB	Nature; FGF-dependent metabolic control of vascular development.; Authors - Pengchun Yu
human	WB	Cell; Role of PFKFB3-Driven Glycolysis in Vessel Sprouting.; Authors - De Bock Katrien K
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li
human	WB,IP	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Circulation; Exercise-Induced Changes in Glucose Metabolism Promote Physiologic Cardiac Growth.; Authors - Andrew A Gibb

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (08-24-2022)

this antibody is working good for westerner blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue and colon cancer cell line

FH

Balawant (Verified Customer) (05-08-2022)

tis antibody is working great at 1:2000 diluton. I have used this antibody from total cells lysate of colonic epithelial cells form human origin.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Colonic epithelial cells

FH

Iram (Verified Customer) (04-13-2022)

Very good signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Colon cancer cells

FH

Susmita (Verified Customer) (03-11-2022)

This antibody works great for me.

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: PC cell line

FH

Hsin-Sheng (Verified Customer) (08-19-2019)

Strong bend intensity at around 60 kD. However, there is a non-specific band near 65-70 kD.

• Applications: Western Blot,
• Primary Antibody Dilution: 1;200
• Cell Tissue Type: H1299

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