Anticorps Recombinant de lapin anti-PFKM

PFKM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241598B2

N° de cat : 84281-5-PBS

Synonymes

241598B2, 6-phosphofructokinase type A, ATP-dependent 6-phosphofructokinase, muscle type, ATP-PFK, EC:2.7.1.11

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Galerie de données de validation

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  WB analysis using 84281-5-RR (same clone as 84281-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84281-5-RR (PFKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 84281-5-RR (same clone as 84281-5-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84281-5-RR (PFKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84281-5-RR (same clone as 84281-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84281-5-RR (PFKM antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84281-5-RR (same clone as 84281-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84281-5-RR (PFKM antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84281-5-RR (same clone as 84281-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PFKM antibody (84281-5-RR, Clone: 241598B2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84281-5-RR (same clone as 84281-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PFKM antibody (84281-5-RR, Clone: 241598B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84281-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01204-3

  Cytometric bead array standard curve of MP01204-3, PFKM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84281-4-PBS. Detection antibody: 84281-5-PBS. Standard: Ag30840. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84281-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84281-5-RR against Human PFKM were performed. The affinity constant is 3.01 nM.

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Informations sur le produit

84281-5-PBS cible PFKM dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PFKM Protéine recombinante Ag30840
• Nom complet: phosphofructokinase, muscle
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC021203
• Symbole du gène: PFKM
• Identification du gène (NCBI): 5213
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM cause glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular weights of 85, 82, and 93 kDa.

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