Anticorps Monoclonal anti-PFKP

PFKP Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H4D4

N° de cat : 68129-1-PBS

Synonymes

1H4D4, 6-phosphofructokinase type C, ATP-dependent 6-phosphofructokinase, platelet type, ATP-PFK, EC:2.7.1.11

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Galerie de données de validation

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  WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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  WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68129-1-Ig (same clone as 68129-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 68129-1-Ig (same clone as 68129-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68129-1-PBS cible PFKP dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PFKP Protéine recombinante Ag25206
• Nom complet: phosphofructokinase, platelet
• Masse moléculaire calculée: 784 aa, 86 kDa
• Poids moléculaire observé: 80-86 kDa
• Numéro d’acquisition GenBank: BC029138
• Symbole du gène: PFKP
• Identification du gène (NCBI): 5214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

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