Anticorps Monoclonal anti-Profilin 1

• Phare
• Validé par KD/KO

Profilin 1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

3A12E8

N° de cat : 67390-1-Ig

Synonymes

PFN1, profilin 1, Profilin I

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Galerie de données de validation

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  WB analysis using 67390-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67390-1-Ig (Profilin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67390-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67390-1-Ig (Profilin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67390-1-Ig

  WB result of Profilin 1 antibody (67390-1-Ig; 1:160000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Profilin 1 transfected HeLa cells.

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  WB analysis of HAP1 using 67390-1-Ig

  HAP1 (WT and PFN1 KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 67390-1-lg  incubated at 1:30,000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human colon cancer using 67390-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67390-1-Ig (Profilin 1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67390-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Profilin 1 antibody (67390-1-Ig, Clone: 3A12E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HAP1 using 67390-1-Ig

  HAP1 WT cells (green outline) and PFN1 KO cells (magenta outline) labelled with a green or a far-red fluorescence dye, respectively. Cells with 4% PFA and stained with 67390-1-lg at 1.0 μg/ml plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, HAP1 cells

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

67390-1-Ig cible Profilin 1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Profilin 1 Protéine recombinante Ag29206
• Nom complet: profilin 1
• Masse moléculaire calculée: 140 aa, 15 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC006768
• Symbole du gène: PFN1
• Identification du gène (NCBI): 5216
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.

Publications

Species	Application	Title
human	IF	Cell Prolif; An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms; Authors - Nan Zhang
human	WB,IF	iScience; Methods to separate nuclear soluble fractions reflecting localizations in living cells; Authors - Yutaka Ogawa
human	WB	Diabetes Metab Syndr Obes; Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway; Authors - Liping Mai
human	WB,IF,IP	F1000Res; The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence; Authors - Riham Ayoubi ; KO Validated