Anticorps Monoclonal anti-PFN2

• Phare
• Validé par KD/KO

PFN2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

2H7C12

N° de cat : 60094-2-Ig

Synonymes

D3S1319E, PFL, PFN2, Prifilin 2, PROF2, profilin 2, Profilin II

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Galerie de données de validation

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  WB analysis of mouse brain using 60094-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 60094-2-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human lung cancer using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of mouse brain using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

60094-2-Ig cible PFN2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PFN2 Protéine recombinante Ag5043
• Nom complet: profilin 2
• Masse moléculaire calculée: 140 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC018049
• Symbole du gène: PFN2
• Identification du gène (NCBI): 5217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PFN2 (profilin 2) is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. The MW of this protein is 15 kDa, and this antibody specially recognises the 15 kDa protein.

Publications

Species	Application	Title
mouse	WB	J Cell Biol; NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis.; Authors - Rui Huang ; KD Validated
human	WB,IHC	Int J Oncol; Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer.; Authors - Hui Zhang
mouse	WB	Front Mol Neurosci; Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice; Authors - Xiaoxia Li
human	WB,IHC	Mol Med; ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.; Authors - Lihong Lu ; KD Validated
human	WB,IHC	J Mammary Gland Biol Neoplasia; Circ_0008500 Knockdown Improves Radiosensitivity and Inhibits Tumorigenesis in Breast Cancer Through the miR-758-3p/PFN2 Axis.; Authors - Deyou Kong
mouse	WB,IF	Biochem Biophys Res Commun; PFN2a, a new partner of RARα in the cytoplasm.; Authors - Dina Andriamoratsiresy