Anticorps Monoclonal anti-PFN2

• Phare
• Validé par KD/KO

PFN2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H7C12

N° de cat : 60094-2-PBS

Synonymes

Prifilin 2, Profilin II, profilin 2, PFL, D3S1319E

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Galerie de données de validation

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  WB analysis of mouse brain using 60094-2-Ig (same clone as 60094-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 60094-2-Ig (same clone as 60094-2-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PFN2 antibody (60094-2-Ig, Clone: 2H7C12 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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Informations sur le produit

60094-2-PBS cible PFN2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PFN2 Protéine recombinante Ag5043
• Nom complet: profilin 2
• Masse moléculaire calculée: 140 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC018049
• Symbole du gène: PFN2
• Identification du gène (NCBI): 5217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PFN2 (profilin 2) is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. The MW of this protein is 15 kDa, and this antibody specially recognises the 15 kDa protein.

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