Anticorps Polyclonal de lapin anti-PGAM1

• Phare
• Validé par KD/KO

PGAM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 16126-1-AP

Synonymes

BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1

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Galerie de données de validation

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  WB analysis using 16126-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16126-1-AP (PGAM1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16126-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16126-1-AP (PGAM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 16126-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16126-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 16126-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16126-1-AP (PGAM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 16126-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16126-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 16126-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules CHO, cellules HEK-293, cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules Raji
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16126-1-AP cible PGAM1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGAM1 Protéine recombinante Ag9110
• Nom complet: phosphoglycerate mutase 1 (brain)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC011678
• Symbole du gène: PGAM1
• Identification du gène (NCBI): 5223
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGAM1(phosphoglycerate mutase 1) is also named as PGAMA,PGAM-B and belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The homodimer PGAM1 is expressed mainly in liver, kidney, brain and overexpressed in a variety of human cancers, including breast carcinoma, colorectal cancer, lung cancer, prostate cancer, oral squamous cell carcinoma, esophageal squamous cell carcinomas and also associated with certain virus infection. PGAM1 could be developed as a useful diagnostic biomarker, as well as a potential therapeutic target for hepatocellular carcinoma (PMID:20403181). This antibody may also recognize PGAM2 and PGAM4 due to the high homology.

Publications

Species	Application	Title
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
human	WB,IF	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB	EMBO J; Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.; Authors - Lihua Wang
human	WB,IP	EMBO Rep; Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.; Authors - Guang Yang ; KD Validated
human	WB	Front Oncol; A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1.; Authors - Chao Hu