• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-PGAM1

PGAM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(39)

N° de cat : 16126-1-AP

Synonymes

PGAM-B, PGAMA, PGAM 1, EC:5.4.2.11, CDABP0006



Applications testées

Résultats positifs en WBcellules A549, cellules CHO, cellules HEK-293, cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules Raji
Résultats positifs en IHChuman normal colon, tissu cérébral humain, tissu de cancer du sein humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules A549,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochimie (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

16126-1-AP cible PGAM1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PGAM1 Protéine recombinante Ag9110
Nom complet phosphoglycerate mutase 1 (brain)
Masse moléculaire calculée 254 aa, 29 kDa
Poids moléculaire observé 29 kDa
Numéro d’acquisition GenBankBC011678
Symbole du gène PGAM1
Identification du gène (NCBI) 5223
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGAM1(phosphoglycerate mutase 1) is also named as PGAMA,PGAM-B and belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The homodimer PGAM1 is expressed mainly in liver, kidney, brain and overexpressed in a variety of human cancers, including breast carcinoma, colorectal cancer, lung cancer, prostate cancer, oral squamous cell carcinoma, esophageal squamous cell carcinomas and also associated with certain virus infection. PGAM1 could be developed as a useful diagnostic biomarker, as well as a potential therapeutic target for hepatocellular carcinoma (PMID:20403181). This antibody may also recognize PGAM2 and PGAM4 due to the high homology.

Protocole

Product Specific Protocols
WB protocol for PGAM1 antibody 16126-1-APDownload protocol
IHC protocol for PGAM1 antibody 16126-1-APDownload protocol
IF protocol for PGAM1 antibody 16126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
humanWB,IF

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
humanWB

Cell Res

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Authors - Linchong Sun
mouseWB

EMBO J

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Authors - Lihua Wang
human,mouseWB

Pharmacol Res

RHBDF1 deficiency suppresses melanoma glycolysis and enhances efficacy of immunotherapy by facilitating glucose-6-phosphate isomerase degradation via TRIM32

Authors - Lei Wang
human,mouseIHC,IF

Int J Biol Macromol

Ubiquitin-conjugating enzyme E2S decreases the sensitivity of glioblastoma cells to temozolomide by upregulating PGAM1 via the interaction with OTUB2

Authors - Lin Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Marco (Verified Customer) (07-04-2024)

It is a very well functioning antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: NRVCMs
{{ptg:RelatedPrimaryAntibodies}}