Anticorps Monoclonal anti-PGAM1

• Phare
• Validé par KD/KO

PGAM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2H2A9

N° de cat : 67470-1-PBS

Synonymes

BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1

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Galerie de données de validation

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  WB analysis using 67470-1-Ig (same clone as 67470-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67470-1-Ig (PGAM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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  WB analysis using 67470-1-Ig (same clone as 67470-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67470-1-Ig (PGAM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67470-1-Ig (same clone as 67470-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67470-1-Ig (PGAM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67470-1-Ig (same clone as 67470-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67470-1-Ig (PGAM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67470-1-Ig (same clone as 67470-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGAM1 antibody (67470-1-Ig, Clone: 2H2A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67470-1-PBS cible PGAM1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PGAM1 Protéine recombinante Ag9250
• Nom complet: phosphoglycerate mutase 1 (brain)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC011678
• Symbole du gène: PGAM1
• Identification du gène (NCBI): 5223
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PGAM1(phosphoglycerate mutase 1) is also named as PGAMA,PGAM-B and belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The homodimer PGAM1 is expressed mainly in liver, kidney, brain and overexpressed in a variety of human cancers, including breast carcinoma, colorectal cancer, lung cancer, prostate cancer, oral squamous cell carcinoma, esophageal squamous cell carcinomas and also associated with certain virus infection. PGAM1 could be developed as a useful diagnostic biomarker, as well as a potential therapeutic target for hepatocellular carcinoma (PMID:20403181). This antibody may also recognize PGAM2 and PGAM4 due to the high homology.

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