Anticorps Polyclonal de lapin anti-PGAM2

PGAM2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15550-1-AP

Synonymes

BPG dependent PGAM 2, PGAM M, PGAM2, PGAMM, Phosphoglycerate mutase 2

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Galerie de données de validation

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  WB analysis using 15550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15550-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15550-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15550-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15550-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa, tissu cardiaque de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque de souris, tissu cérébral de souris, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15550-1-AP cible PGAM2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGAM2 Protéine recombinante Ag7908
• Nom complet: phosphoglycerate mutase 2 (muscle)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC001904
• Symbole du gène: PGAM2
• Identification du gène (NCBI): 5224
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody may also recognize PGAM1 and PGAM4 due to the high homology.

Publications

Species	Application	Title
human,mouse	IHC,IF	EMBO Rep; METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cells; Authors - Yichao Hou
pig	WB	Sci Rep; Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.; Authors - Liangliang Fu
human	WB	J Proteomics; Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility.; Authors - Yueshuai Guo
mouse	WB	Sci Adv; Lactate metabolism is essential in early-onset mitochondrial myopathy; Authors - Zhenkang Chen
mouse	WB	Redox Biol; Unbalanced redox status network as an early pathological event in congenital cataracts; Authors - Eloy Bejarano
human,mouse	WB,IP	Cell Res; Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate; Authors - Yu-Qing Wu