Anticorps Polyclonal de lapin anti-PGAM2

PGAM2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 15550-1-PBS

Synonymes

BPG dependent PGAM 2, BPG-dependent PGAM 2, EC:5.4.2.11, Muscle-specific phosphoglycerate mutase, PGAM M

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Galerie de données de validation

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  WB analysis using 15550-1-AP (same clone as 15550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15550-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15550-1-PBS cible PGAM2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGAM2 Protéine recombinante Ag7908
• Nom complet: phosphoglycerate mutase 2 (muscle)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC001904
• Symbole du gène: PGAM2
• Identification du gène (NCBI): 5224
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody may also recognize PGAM1 and PGAM4 due to the high homology.

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