Anticorps Monoclonal anti-PGAM5

PGAM5 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C8B9

N° de cat : 68116-1-PBS

Synonymes

1C8B9, Bcl-XL-binding protein v68, BXLBv68, EC:3.1.3.16, Serine/threonine-protein phosphatase PGAM5, mitochondrial

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Galerie de données de validation

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  WB analysis using 68116-1-Ig (same clone as 68116-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 68116-1-Ig (same clone as 68116-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 68116-1-Ig (same clone as 68116-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68116-1-Ig (same clone as 68116-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68116-1-Ig (same clone as 68116-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68116-1-PBS cible PGAM5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PGAM5 Protéine recombinante Ag28195
• Nom complet: phosphoglycerate mutase family member 5
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC008196
• Symbole du gène: PGAM5
• Identification du gène (NCBI): 192111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.

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