Anticorps Recombinant de lapin anti-PGCP

PGCP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251059H3

N° de cat : 86405-1-RR

Synonymes

Carboxypeptidase Q, CPQ, EC:3.4.17.-, LCH1, Lysosomal dipeptidase

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Galerie de données de validation

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  WB analysis using 86405-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86405-1-RR (PGCP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Negative control: mouse brain tissue

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  IF Staining of L02 using 86405-1-RR

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using PGCP antibody (86405-1-RR, Clone: 251059H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86405-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86405-1-RR against Human PGCP were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu rénal de rat,
• Résultats positifs en IF/ICC: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86405-1-RR cible PGCP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PGCP Protéine recombinante Ag9770
• Nom complet: plasma glutamate carboxypeptidase
• Masse moléculaire calculée: 472 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC020689
• Symbole du gène: PGCP
• Identification du gène (NCBI): 10404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGCP(plasma glutamate carboxypeptidase) is also named as LCH1,CPQ and belongs to the peptidase M28 family. It may play an important role in the hydrolysis of circulating peptides. It catalyzes more efficiently the hydrolysis of dipeptides with unsubstituted terminals into amino acids. It is a proteinase that acts on the unsubstituted N- and C-termini of dipeptides. It has been suggested that this PGCP is involved in the release of thyroxine(PMID:20951214). Human PGCP is a metallopeptidase with five potential glycosylation sites, whose activity depends on dimerizationand it can be detected a band of 65-72kd in FRTL-5 cells which is pro-PGCP(PMID:22127294). It can be N-glycosylated(PMID:10206990). It has the pro-form of 60-65 kDa and mature form of 55 kDa(PMID:20951214).

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