Anticorps Monoclonal anti-PGD

PGD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A10G4

N° de cat : 67916-1-PBS

Synonymes

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

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Galerie de données de validation

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  WB analysis using 67916-1-Ig (same clone as 67916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67916-1-PBS in a different storage buffer formulation.

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  WB analysis using 67916-1-Ig (same clone as 67916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67916-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67916-1-Ig (same clone as 67916-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PGD antibody (67916-1-Ig, Clone: 2A10G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67916-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67916-1-PBS cible PGD dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PGD Protéine recombinante Ag6871
• Nom complet: phosphogluconate dehydrogenase
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 53 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC000368
• Symbole du gène: PGD
• Identification du gène (NCBI): 5226
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)

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