Anticorps Polyclonal de lapin anti-HPGDS

HPGDS Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 22522-1-AP

Synonymes

Glutathione S transferase, GST class sigma, GSTS, H PGDS, HPGDS, PGDS, Prostaglandin H2 D isomerase, PTGDS2

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Galerie de données de validation

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  WB analysis using 22522-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22522-1-AP (HPGDS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 22522-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22522-1-AP (HPGDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22522-1-AP (HPGDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22522-1-AP (HPGDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22522-1-AP (HPGDS antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22522-1-AP (HPGDS antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu splénique de rat, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

22522-1-AP cible HPGDS dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HPGDS Protéine recombinante Ag18290
• Nom complet: prostaglandin D2 synthase, hematopoietic
• Masse moléculaire calculée: 199 aa, 23 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC020734
• Symbole du gène: PGDS
• Identification du gène (NCBI): 27306
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hematopoietic prostaglandin D synthase (HPGDS) is expressed in human Th2 lymphocytes, antigen-presenting cells, mast cells and microglia. HPGDS converts PGH2 into PGD2, a mediator thought to play a pivotal role in airway allergy and inflammatory processes (PMID: 16624958). HPGDS deficiency is a critical factor that impedes cutaneous wound healing in diabetes. Overexpressing Hpgds in ADSCs could be an effective way to accelerate DW healing by alleviating inflammatory cells recruitment and increasing M2 polarization in the proliferation phase (PMID: 35922870). HPGDS is a cytosolic homodimer of 26 kDa subunits which reveal a well-defined active site, enabling a structure-based design of inhibitors (PMID: 24900177).

Publications

Species	Application	Title
mouse	IF	Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong
human	WB	EMBO Mol Med; Tranilast directly targets NLRP3 to treat inflammasome-driven diseases.; Authors - Yi Huang
mouse	WB	Molecules; Integrative Analysis of Proteomics and Metabolism Reveals the Potential Roles of Arachidonic Acid Metabolism in Hypoxia Response in Mouse Spleen; Authors - Yujing Guo