Anticorps Polyclonal de lapin anti-PGK1

• Phare
• Validé par KD/KO

PGK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(95)

N° de cat : 17811-1-AP

Synonymes

Cell migration-inducing gene 10 protein, EC:2.7.2.3, OK/SW-cl.110, PGKA, PRP 2

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Galerie de données de validation

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  WB analysis using 17811-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17811-1-AP

  WB result of PGK1 antibody (17811-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGK1 transfected HeLa cells.

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  WB analysis of HepG2 using 17811-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17811-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of SW 1990 using 17811-1-AP

  IP result of anti-PGK1 (IP:17811-1-AP, 4ug; Detection:17811-1-AP 1:2000) with SW 1990 cells lysate 2800 ug.

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  IP experiment of HeLa using 17811-1-AP

  IP result of anti-PGK1 (IP:17811-1-AP, 4ug; Detection:17811-1-AP 1:10000) with HeLa cells lysate 980 ug.

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  IHC staining of human colon cancer using 17811-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17811-1-AP (PGK1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17811-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17811-1-AP (PGK1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 17811-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PGK1 antibody (17811-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 17811-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGK1 antibody (17811-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, cellules PC-12, cellules PC-3, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules SW-1990, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 82 publications below
• IHC: See 20 publications below
• IF: See 7 publications below
• IP: See 6 publications below
• CoIP: See 5 publications below

Informations sur le produit

17811-1-AP cible PGK1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGK1 Protéine recombinante Ag12119
• Nom complet: phosphoglycerate kinase 1
• Masse moléculaire calculée: 417 aa, 45 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC103752
• Symbole du gène: PGK1
• Identification du gène (NCBI): 5230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). PGK1 has 2 isoforms with the molecular mass of 45 kDa and 41 kDa.

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB,IP,IF	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	Nat Commun; Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1α pathway suppression.; Authors - Mei-Lin Weng
human	WB,IHC,IF,CoIP	Exp Hematol Oncol; Downregulation of pro-surfactant protein B contributes to the recurrence of early-stage non-small cell lung cancer by activating PGK1-mediated Akt signaling; Authors - Hao Luo

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