Anticorps Monoclonal anti-PGK1

PGK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1B2F3

N° de cat : 68035-1-Ig

Synonymes

PRP 2, PGKA, OK/SW-cl.110, EC:2.7.2.3, Cell migration-inducing gene 10 protein

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Galerie de données de validation

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  WB analysis using 68035-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68035-1-Ig (PGK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 68035-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 68035-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PGK1 antibody (68035-1-Ig, Clone: 1B2F3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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  IF Staining of HepG2 using 68035-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGK1 antibody (68035-1-Ig, Clone: 1B2F3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules Jurkat, cellules LNCaP, cellules NCCIT, cellules PC-3, tissu plaquettaire humain, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal de rat, tissu rénal de souris, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

68035-1-Ig cible PGK1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PGK1 Protéine recombinante Ag12099
• Nom complet: phosphoglycerate kinase 1
• Masse moléculaire calculée: 417 aa, 45 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC103752
• Symbole du gène: PGK1
• Identification du gène (NCBI): 5230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3- phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei. PGK1 has 2 isoforms with the molecular mass of 41 and 45 kDa (PMID: 31815035, PMID: 31911580).

Publications

Species	Application	Title
human	WB	Transl Oncol; Tetraarsenic tetrasulfide triggers ROS-induced apoptosis and ferroptosis in B-cell acute lymphoblastic leukaemia by targeting HK2; Authors - Wenke Bai
	WB	J Biol Chem; Hypoxia-inducible factor-1 alpha modulates muscle growth and the molting process through its regulation of glycolysis in Neocaridina davidi; Authors - Ran Li

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