Anticorps Polyclonal de lapin anti-PGK2

PGK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13686-1-AP

Synonymes

PGK 2, PGK2, PGKB, PGKPS, phosphoglycerate kinase 2

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Galerie de données de validation

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  WB analysis of mouse testis using 13686-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13686-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13686-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13686-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13686-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13686-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse testis using 13686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 13686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HeLa, cellules HepG2, tissu cardiaque humain, tissu cérébral de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

13686-1-AP cible PGK2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGK2 Protéine recombinante Ag4553
• Nom complet: phosphoglycerate kinase 2
• Masse moléculaire calculée: 417 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC038843
• Symbole du gène: PGK2
• Identification du gène (NCBI): 5232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.

Publications

Species	Application	Title
mouse	WB	Chemosphere; Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.; Authors - Yongli Han
mouse	WB	Cell Physiol Biochem; Effect of Type I Diabetes on the Proteome of Mouse Oocytes.; Authors - Guangjian Jiang
sheep	WB	Theriogenology; Characterization of GALNTL5 gene sequence and expression in ovine testes and sperm.; Authors - Xiaolei Yao
mouse	WB	Nature; A male germ-cell-specific ribosome controls male fertility; Authors - Huiling Li
human	WB	Cell Res; Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate; Authors - Yu-Qing Wu ; KD Validated