Anticorps Monoclonal anti-PGM1

PGM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G5A8

N° de cat : 66105-1-PBS

Synonymes

2G5A8, CDG1T, EC:5.4.2.2, GSD14, Phosphoglucomutase-1

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Galerie de données de validation

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  WB analysis using 66105-1-Ig (same clone as 66105-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66105-1-Ig (PGM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGM1 antibody (66105-1-Ig, Clone: 2G5A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66105-1-PBS cible PGM1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PGM1 Protéine recombinante Ag7294
• Nom complet: phosphoglucomutase 1
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC001756
• Symbole du gène: PGM1
• Identification du gène (NCBI): 5236
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

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