Anticorps Recombinant de lapin anti-PGM5

PGM5 Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230206C7

N° de cat : 82965-1-PBS

Synonymes

230206C7, Aciculin, PGMRP

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Galerie de données de validation

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  WB analysis using 82965-1-RR (same clone as 82965-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82965-1-RR (PGM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82965-1-RR (same clone as 82965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82965-1-RR (PGM5 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82965-1-RR (same clone as 82965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82965-1-RR (PGM5 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 82965-1-RR (same clone as 82965-1-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human PGM5 (82965-1-RR, Clone:230206C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82965-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82965-1-RR against Human PGM5 were performed. The affinity constant is 0.719 nM.

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Informations sur le produit

82965-1-PBS cible PGM5 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PGM5 Protéine recombinante Ag34399
• Nom complet: phosphoglucomutase 5
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: NM_021965
• Symbole du gène: PGM5
• Identification du gène (NCBI): 5239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoglucomutase-like protein 5 (also known as aciculin) is an enzyme encoded by the PGM5 gene. Gene functional studies show that PGM5 is similar to PGM1 but lacks enzymatic activity. PGM5 is tightly associated with the actin cytoskeleton and has primarily been investigated as an adhesion protein. It also functions as a cytoskeletal component of cell-matrix and cell-cell contacts in muscle and non-muscle cells. (PMID: 35819729)

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