Anticorps Monoclonal anti-PGRMC2

• Phare
• Validé par KD/KO

PGRMC2 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5F10D7

N° de cat : 60249-1-PBS

Synonymes

5F10D7, DG6, Membrane-associated progesterone receptor component 2, PMBP

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Galerie de données de validation

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  WB analysis using 60249-1-Ig (same clone as 60249-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60249-1-Ig (PGRMC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60249-1-Ig (PGRMC2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60249-1-Ig (PGRMC2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

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  IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using PGRMC2 antibody (60249-1-Ig, Clone: 5F10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

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  IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using PGRMC2 antibody (60249-1-Ig, Clone: 5F10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 60249-1-Ig (same clone as 60249-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PGRMC2 (60249-1-Ig, Clone:5F10D7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60249-1-PBS cible PGRMC2 dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PGRMC2 Protéine recombinante Ag20077
• Nom complet: progesterone receptor membrane component 2
• Masse moléculaire calculée: 223 aa, 24 kDa
• Poids moléculaire observé: 24 kDa, 28 kDa
• Numéro d’acquisition GenBank: BC016692
• Symbole du gène: PGRMC2
• Identification du gène (NCBI): 10424
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this monoclonal antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494).

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