Anticorps Recombinant de lapin anti-PHD1

PHD1 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251511G2

N° de cat : 86614-2-PBS

Synonymes

EGLN2, EC:1.14.11.-, EC:1.14.11.29, EIT6, EIT-6

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Galerie de données de validation

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  WB analysis using 86614-2-RR (same clone as 86614-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86614-2-RR (EGLN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86614-2-PBS in a different storage buffer formulation.

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  WB analysis using 86614-2-RR (same clone as 86614-2-PBS)

  Rat testis tissue were subjected to SDS PAGE followed by western blot with 86614-2-RR (EGLN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86614-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86614-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86614-2-RR against Human PHD1 were performed. The affinity constant is 0.587 nM.

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Informations sur le produit

86614-2-PBS cible PHD1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PHD1 Protéine recombinante Ag3616
• Nom complet: egl nine homolog 2 (C. elegans)
• Masse moléculaire calculée: 407 aa, 44 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC036051
• Symbole du gène: PHD1
• Identification du gène (NCBI): 112398
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHD1, also named as EGLN2, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.

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