Anticorps Monoclonal anti-PHEX

PHEX Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D4B2

N° de cat : 66934-1-PBS

Synonymes

1D4B2, EC:3.4.24.-, HPDR, HYP1, PEX

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Galerie de données de validation

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  WB analysis of HEK-293 using 66934-1-Ig (same clone as 66934-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

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  WB analysis of rat lung using 66934-1-Ig (same clone as 66934-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

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  WB analysis of pig lung using 66934-1-Ig (same clone as 66934-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66934-1-Ig (same clone as 66934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66934-1-Ig (PHEX antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66934-1-PBS cible PHEX dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PHEX Protéine recombinante Ag20290
• Nom complet: phosphate regulating endopeptidase homolog, X-linked
• Masse moléculaire calculée: 749 aa, 86 kDa
• Poids moléculaire observé: 76-80 kDa, 95-100 kDa
• Numéro d’acquisition GenBank: BC105059
• Symbole du gène: PHEX
• Identification du gène (NCBI): 5251
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660). Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349).

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