Anticorps Polyclonal de lapin anti-PHF12

PHF12 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 24485-1-AP

Synonymes

KIAA1523, PF1, PHD factor 1, PHD finger protein 12, PHF12

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Galerie de données de validation

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  WB analysis of mouse brain using 24485-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24485-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 24485-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 24485-1-AP

  IP result of anti-PHF12 (IP:24485-1-AP, 4ug; Detection:24485-1-AP 1:500) with mouse brain tissue lysate 3600ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

24485-1-AP cible PHF12 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHF12 Protéine recombinante Ag18471
• Nom complet: PHD finger protein 12
• Masse moléculaire calculée: 1004 aa, 110 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC121043
• Symbole du gène: PHF12
• Identification du gène (NCBI): 57649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Plant homeodomain [PHD] zinc finger protein 12 (PHF12), also known Pf1, is a member of the PHD zinc finger family of proteins. Pf1 associates with a chromatin-interacting protein complex comprised of MRG15, Sin3B, and histone deacetylase 1 (HDAC1) that functions as a transcriptional modulator.

Publications

Species	Application	Title
human	WB	EMBO Rep; MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.; Authors - Gongwei Wu
human	WB	J Transl Med; PHF12 regulates HDAC1 to promote tumorigenesis via EGFR/AKT signaling pathway in non-small cell lung cancer; Authors - Yiru Kong ; KD Validated

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