Anticorps Polyclonal de lapin anti-PHF14

• Phare
• Validé par KD/KO

PHF14 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 24787-1-AP

Synonymes

KIAA0783, PHD finger protein 14

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Galerie de données de validation

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  WB analysis of HeLa using 24787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24787-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 24787-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24787-1-AP

  IP result of anti-PHF14 (IP:24787-1-AP, 4ug; Detection:24787-1-AP 1:500) with HEK-293 cells lysate 1200ug.

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  IHC staining of human stomach cancer using 24787-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24787-1-AP (PHF14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 24787-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24787-1-AP (PHF14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 24787-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24787-1-AP (PHF14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MDA-MB-453s
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below

Informations sur le produit

24787-1-AP cible PHF14 dans les applications de WB, IHC, IF, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHF14 Protéine recombinante Ag20542
• Nom complet: PHD finger protein 14
• Masse moléculaire calculée: 888 aa, 100 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC152414
• Symbole du gène: PHF14
• Identification du gène (NCBI): 9678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHF14 also named as KIAA0783 or PHD finger protein 14 is 888 amino acid protein, which contains 2 PHD-type znic fingers. PHF14 localizes in nucleus and may be involved in the regulation of transcription, DNA dependent. The function of PHF14 is still non-known.

Publications

Species	Application	Title
mouse,human	WB,CoIP,ChIP	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	WB	Elife; Integrated analysis of H2A.Z isoforms function reveals a complex interplay in gene regulation.; Authors - Assala Lamaa
human	WB,IHC	Biomed Res Int; PHF14 Promotes Cell Proliferation and Migration through the AKT and ERK1/2 Pathways in Gastric Cancer Cells.; Authors - Yuzu Zhao ; KD Validated
human	WB,CoIP,ChIP	Nucleic Acids Res; The high mobility group protein HMG20A cooperates with the histone reader PHF14 to modulate TGFβ and Hippo pathways; Authors - Elena Gómez-Marín ; KD Validated
human	WB,IHC,IF,ChIP	Cell Discov; PHF14 enhances DNA methylation of SMAD7 gene to promote TGF-β-driven lung adenocarcinoma metastasis; Authors - Han Tian
human	WB	Mol Cancer Res; SP4 facilitates esophageal squamous cell carcinoma progression by activating PHF14 transcription and Wnt/β-Catenin signaling; Authors - Li Wei ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jose C. (Verified Customer) (10-14-2019)

The antibody gives good results in WB, IP and co-IP. We have verified by siRNA that the identified band correspond to PHF14.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: MDA-MB-231, HEK293T and MCF7

PHF14 Antibody Western Blot,Immunoprecipitation, validation (1/500 dilution) in MDA-MB-231, HEK293T and MCF7 (Cat no:24787-1-AP)

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