Anticorps Polyclonal de lapin anti-PHF17

PHF17 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 15032-1-AP

Synonymes

Jade family PHD finger protein 1, JADE1, PHF 17, Protein Jade-1

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Galerie de données de validation

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  WB analysis of HeLa using 15032-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15032-1-AP (PHF17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15032-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15032-1-AP (PHF17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

15032-1-AP cible PHF17 dans les applications de WB, IHC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHF17 Protéine recombinante Ag4290
• Nom complet: PHD finger protein 17
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 65 kDa, 100-110 kDa
• Numéro d’acquisition GenBank: BC032376
• Symbole du gène: PHF17
• Identification du gène (NCBI): 79960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHF17 was identified as a protein partner of the von Hippel-Lindau tumor suppressor pVHL. It is one component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It can act as a transcriptional coactivator that promote acetylation of nucleosomal histone H4 by KAT5. In addition, it ubiquitinates both phosphorylated and non-phosphorylated β-catenin and therefore regulates canonical Wnt signaling in both Wnt-off and Wnt-on phases. PHF17 exist several isoforms with molecular weight 100-110 and 55-65 kDa.

Publications

Species	Application	Title
human	WB,IP,IHC	Acta Neuropathol; Genome-wide association study and functional validation implicates JADE1 in tauopathy.; Authors - Kurt Farrell
human	WB	J Biol Chem; The ciliary protein nephrocystin-4 translocates the canonical Wnt regulator Jade-1 to the nucleus to negatively regulate β-catenin signaling.; Authors - Borgal Lori L
human	WB,IP	J Biol Chem; Casein Kinase 1 Alpha Phosphorylates the Wnt-Regulator Jade-1 and Modulates its Activity.; Authors - Lori Borgal
human	WB	Cell Cycle; Jade-1S phosphorylation induced by CK1α contributes to cell cycle progression.; Authors - Lori Borgal
human	WB	J Toxicol Sci; Involvement of microRNA-4680-3p against phenytoin-induced cell proliferation inhibition in human palate cells; Authors - Yosuke Tsukiboshi
mouse,human	WB,CoIP	bioRxiv; Jade1 and the HBO1 complex are spatial-selective cofactors of Oct4; Authors - Yifan Wu

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