Anticorps Polyclonal de lapin anti-PHF17

• Phare
• Validé par KD/KO

PHF17 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28472-1-PBS

Synonymes

Jade family PHD finger protein 1, JADE1, PHF 17, Protein Jade-1

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Galerie de données de validation

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  WB analysis using 28472-1-AP (same clone as 28472-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 28472-1-AP (same clone as 28472-1-PBS)

  WB result of PHF17 antibody (28472-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF17 transfected HepG2 cells. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 28472-1-AP (same clone as 28472-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

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  WB analysis using 28472-1-AP (same clone as 28472-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28472-1-AP (same clone as 28472-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF17 antibody (28472-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28472-1-PBS cible PHF17 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHF17 Protéine recombinante Ag29675
• Nom complet: PHD finger protein 17
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 65 kDa, 100-110 kDa
• Numéro d’acquisition GenBank: BC032376
• Symbole du gène: PHF17
• Identification du gène (NCBI): 79960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHF17, also named as JADE1 and KIAA1807, belongs to the JADE family. PHF17 codes for the Jade-1 protein. This protein interacts with histone acetyltransferase HBO1 and promotes histone acetylation in chromatin to regulate DNA replication and gene transcription. PHF17 is mostly expressed in the small intestine, prostate, mammary gland and kidney. PHF17 is involved in mitosis, meiosis and tumor suppression (PMID: 28134766). PHF17 has 3 isoforms with the molecular mass of 58 and 94-96 kDa. With modification, the MW of PHF17 may be migrated to about 65 kDa and 100-110 kDa.

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