Anticorps Recombinant de lapin anti-PHF19

PHF19 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241047D7

N° de cat : 83892-3-PBS

Synonymes

241047D7, hPCL3, MTF2L1, PCL3, PHD finger protein 19

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Galerie de données de validation

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  WB analysis using 83892-3-RR (same clone as 83892-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83892-3-RR (PHF19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83892-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83892-3-RR (same clone as 83892-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PHF19 Recombinant antibody (83892-3-RR, Clone:241047D7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83892-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00868-2

  Cytometric bead array standard curve of MP00868-2, PHF19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83892-5-PBS. Detection antibody: 83892-3-PBS. Standard: Ag2511. Range: 0.313-40 ng/mL

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  Affinity and Kinetic Characterization of 83892-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83892-3-RR against Human PHF19 were performed. The affinity constant is 0.806 nM.

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Informations sur le produit

83892-3-PBS cible PHF19 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PHF19 Protéine recombinante Ag2511
• Nom complet: PHD finger protein 19
• Masse moléculaire calculée: 580 aa, 63 kDa
• Poids moléculaire observé: 62-66 kDa
• Numéro d’acquisition GenBank: BC022374
• Symbole du gène: PHF19
• Identification du gène (NCBI): 26147
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.

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