Anticorps Polyclonal de lapin anti-PHF23

• Phare
• Validé par KD/KO

PHF23 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25862-1-PBS

Synonymes

PDH-containing protein JUNE-1, hJUNE-1b, hJUNE 1b

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Galerie de données de validation

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  WB analysis using 25862-1-AP (same clone as 25862-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25862-1-AP (PHF23 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25862-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 25862-1-AP (same clone as 25862-1-PBS)

  WB result of PHF23 antibody (25862-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF23 transfected HeLa cells. This data was developed using the same antibody clone with 25862-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 25862-1-AP (same clone as 25862-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PHF23 antibody (25862-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25862-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25862-1-PBS cible PHF23 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHF23 Protéine recombinante Ag23044
• Nom complet: PHD finger protein 23
• Masse moléculaire calculée: 403 aa, 44 kDa
• Poids moléculaire observé: ~65 kDa
• Numéro d’acquisition GenBank: BC002509
• Symbole du gène: PHF23
• Identification du gène (NCBI): 79142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHD finger protein 23 (PHF23), also known as hJUNE-1b, belongs to the PHF23 family. As a putative epigenetic reader, the PHD domain of PHF23 specifically binds to tri-methylated lysine 4 of histone H3 (H3K4me3). PHF23 is widely expressed in human tissues and various cell lines and exerts a negative effect on autophagy by promoting the degradation of E3 ligase LRSAM1 (leucine-rich repeat and sterile alpha motif containing 1) (PMID: 32978226, 32380078). The observed molecular weight of PHF23 is ~65 kDa, which may be caused by post-translational modification. The specificity of this antibody was demonstrated by siRNA-mediated knockdown of target protein.

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