Anticorps Polyclonal de lapin anti-PHF5A

• Phare
• Validé par KD/KO

PHF5A Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 15554-1-AP

Synonymes

bK223H9.2, INI, PHD finger protein 5A, PHD finger-like domain protein 5A, PHD finger-like domain-containing protein 5A

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Galerie de données de validation

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  WB analysis using 15554-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15554-1-AP (PHF5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15554-1-AP

  WB result of PHF5A antibody (15554-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF5A transfected HEK-293 cells.

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  IP experiment of HeLa using 15554-1-AP

  IP result of anti-PHF5A (IP:15554-1-AP, 4ug; Detection:15554-1-AP 1:2000) with HeLa cells lysate 2520 ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below

Informations sur le produit

15554-1-AP cible PHF5A dans les applications de WB, IHC, IF, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHF5A Protéine recombinante Ag7918
• Nom complet: PHD finger protein 5A
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12-14 kDa
• Numéro d’acquisition GenBank: BC007321
• Symbole du gène: PHF5A
• Identification du gène (NCBI): 84844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PHD finger-like domain protein 5a (PHF5A) is ubiquitously expressed and is located in the nucleus. The gene Phf5a and its human counterpart PHF5A (former name Ini) are located on rat chromosome 7 (RNO7q34) and human chromosome 22 (HSA2213q2), respectively. The rat gene consists of four exons, while the human gene have five exons. Both genes encode a highly conserved protein of 110 amino acids that contains a PHD finger domain.(PMID: 23675859) It acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha and is involved in pre-mRNA splicing.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.; Authors - Alexandros Strikoudis ; KO Validated
human	WB,IP,IF,ChIP	Gastroenterology; RNA Polymerase II-Associated Factor 1 Regulates Stem Cell Features of Pancreatic Cancer Cells, Independently of the PAF1 Complex, via Interactions With PHF5A and DDX3.; Authors - Saswati Karmakar
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
mouse,human	WB,CoIP,ChIP	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
		Genes Dev; The cryo-EM structure of the SF3b spliceosome complex bound to a splicing modulator reveals a pre-mRNA substrate competitive mechanism of action.; Authors - Lorenzo I Finci
human	WB	Nat Commun; Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.; Authors - Teng Teng

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