Anticorps Polyclonal de lapin anti-PHGDH

• Phare
• Validé par KD/KO

PHGDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(70)

N° de cat : 14719-1-AP

Synonymes

EC:1.1.1.399, EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 3PGDH

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Galerie de données de validation

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  WB analysis of HEK-293 using 14719-1-AP

  WB result of PHGDH antibody (14719-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHGDH transfected HEK-293 cells.

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  WB analysis using 14719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14719-1-AP (PHGDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14719-1-AP (PHGDH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14719-1-AP

  IP result of anti-PHGDH (IP:14719-1-AP, 4ug; Detection:14719-1-AP 1:10000) with HeLa cells lysate 1880 ug.

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  IHC staining of human lymphoma using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14719-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PHGDH antibody (14719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, cellules MDA-MB-453s, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de lymphome humain, tissu cérébral de souris, tissu d'hyperplasie mammaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 14 publications below
• WB: See 58 publications below
• IHC: See 20 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

14719-1-AP cible PHGDH dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHGDH Protéine recombinante Ag6445
• Nom complet: phosphoglycerate dehydrogenase
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC000303
• Symbole du gène: PHGDH
• Identification du gène (NCBI): 26227
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).

Publications

Species	Application	Title
mouse	IF,ChIP	Cell; Transcriptional regulation by PHGDH drives amyloid pathology in Alzheimer's disease; Authors - Junchen Chen
human,mouse	IHC	Cell Metab; PHGDH expression increases with progression of Alzheimer's disease pathology and symptoms.; Authors - Xu Chen
mouse	WB	Nat Immunol; PERK is a critical metabolic hub for immunosuppressive function in macrophages.; Authors - Lydia N Raines
human,mouse	IHC,CoIP	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
mouse	WB,IF	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB,IHC	Mol Cancer; CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner; Authors - Xin Liu

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