Anticorps Polyclonal de lapin anti-PHIP

PHIP Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20933-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 20933-1-AP (same clone as 20933-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 20933-1-AP (same clone as 20933-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

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  IP experiment of A375 using 20933-1-AP (same clone as 20933-1-PBS)

  IP result of anti-PHIP (IP:20933-1-AP, 4ug; Detection:20933-1-AP 1:3000) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 20933-1-AP (same clone as 20933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 20933-1-AP (PHIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20933-1-PBS cible PHIP dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHIP Protéine recombinante Ag14968
• Nom complet: pleckstrin homology domain interacting protein
• Masse moléculaire calculée: 1821 aa, 207 kDa
• Poids moléculaire observé: 207 kDa
• Numéro d’acquisition GenBank: BC008909
• Symbole du gène: PHIP
• Identification du gène (NCBI): 55023
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.

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