Anticorps Recombinant de lapin anti-PHKG2

PHKG2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240643E1

N° de cat : 83663-2-PBS

Synonymes

240643E1, EC:2.7.11.19, GSD9C, PHK gamma T, PHK-gamma-LT

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Galerie de données de validation

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  WB analysis using 83663-2-RR (same clone as 83663-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83663-2-RR (PHKG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83663-2-RR (same clone as 83663-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83663-2-RR (PHKG2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83663-2-RR (same clone as 83663-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83663-2-RR (PHKG2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 83663-2-RR (same clone as 83663-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHKG2 antibody (83663-2-RR, Clone: 240643E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 83663-2-RR (same clone as 83663-2-PBS)

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug PHKG2 Recombinant antibody (83663-2-RR, Clone:240643E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83663-2-RR (same clone as 83663-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PHKG2 Recombinant antibody (83663-2-RR, Clone:240643E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00632-2

  Cytometric bead array standard curve of MP00632-2, PHKG2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83663-4-PBS. Detection antibody: 83663-2-PBS. Standard: Ag6829. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83663-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83663-2-RR against Human PHKG2 were performed. The affinity constant is 1.90 nM.

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Informations sur le produit

83663-2-PBS cible PHKG2 dans les applications de WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PHKG2 Protéine recombinante Ag6829
• Nom complet: phosphorylase kinase, gamma 2 (testis)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC002541
• Symbole du gène: PHKG2
• Identification du gène (NCBI): 5261
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHKG2 (phosphorylase kinase catalytic subunit gamma 2), it is expected to be located in the cytoplasm, which is enriched in the testis. Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. It may regulates glycogeneolysis in the testis. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. The molecular weight of PHKG2 is 46 kDa.

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