Anticorps Polyclonal de lapin anti-PHLDA1

• Phare
• Validé par KD/KO

PHLDA1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18263-1-PBS

Synonymes

Apoptosis-associated nuclear protein, DT1P1B11, PHRIP, Pleckstrin homology-like domain family A member 1, PQ-rich protein

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Galerie de données de validation

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  WB analysis using 18263-1-AP (same clone as 18263-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 18263-1-AP (same clone as 18263-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 18263-1-AP (same clone as 18263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18263-1-AP (PHLDA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 18263-1-AP (same clone as 18263-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using PHLDA1 antibody (18263-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18263-1-PBS cible PHLDA1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHLDA1 Protéine recombinante Ag13125
• Nom complet: pleckstrin homology-like domain, family A, member 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC018929
• Symbole du gène: PHLDA1
• Identification du gène (NCBI): 22822
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHLDA1, also known as PHRIP and TDAG51, is a multifunctional protein involved in various biological processes. It can induce apoptosis in various cell types, including T cells, hippocampal cells, endothelial cells, melanoma cells, and mouse embryonic fibroblasts(PMID: 30207029). PHLDA1 plays a role in inhibiting growth factor signaling and has been implicated in tumor suppression through its ability to repress Akt activity by binding to phosphatidylinositol (PIP) lipids(PMID: 36142223).

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