Anticorps Recombinant de lapin anti-PHLDA1

PHLDA1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

243156B7

N° de cat : 85783-1-RR

Synonymes

Apoptosis-associated nuclear protein, DT1P1B11, PHRIP, Pleckstrin homology-like domain family A member 1, PQ-rich protein

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Galerie de données de validation

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  WB analysis using 85783-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85783-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85783-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85783-1-RR against Human PHLDA1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules A375, cellules BxPC-3, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85783-1-RR cible PHLDA1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PHLDA1 Protéine recombinante Ag13125
• Nom complet: pleckstrin homology-like domain, family A, member 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC018929
• Symbole du gène: PHLDA1
• Identification du gène (NCBI): 22822
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHLDA1, also known as PHRIP and TDAG51, is a multifunctional protein involved in various biological processes. It can induce apoptosis in various cell types, including T cells, hippocampal cells, endothelial cells, melanoma cells, and mouse embryonic fibroblasts(PMID: 30207029). PHLDA1 plays a role in inhibiting growth factor signaling and has been implicated in tumor suppression through its ability to repress Akt activity by binding to phosphatidylinositol (PIP) lipids(PMID: 36142223).

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