Anticorps Monoclonal anti-PHLPP1

PHLPP1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E8E6

N° de cat : 67640-1-PBS

Synonymes

PLEKHE1, 2E8E6, EC:3.1.3.16, hSCOP, KIAA0606

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Galerie de données de validation

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  WB analysis of mouse brain using 67640-1-Ig (same clone as 67640-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67640-1-Ig (same clone as 67640-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67640-1-Ig (same clone as 67640-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67640-1-Ig (same clone as 67640-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67640-1-PBS cible PHLPP1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PHLPP1 Protéine recombinante Ag18982
• Nom complet: PH domain and leucine rich repeat protein phosphatase
• Masse moléculaire calculée: 1717 aa, 184 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC126277
• Symbole du gène: PHLPP
• Identification du gène (NCBI): 23239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP1 beta is 185 kDa.

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