Anticorps Polyclonal de lapin anti-PHOX2B

PHOX2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 25276-1-AP

Synonymes

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, Paired-like homeobox 2B, PMX2B

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Galerie de données de validation

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  WB analysis of Neuro-2a using 25276-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25276-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25276-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of neuroblastoma using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of neuroblastoma using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of SH-SY5Y using 25276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PHOX2B antibody (25276-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of SH-SY5Y using 25276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 25276-1-AP (PHOX2B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de neuroblastome, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

25276-1-AP cible PHOX2B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHOX2B Protéine recombinante Ag13196
• Nom complet: paired-like homeobox 2b
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC017199
• Symbole du gène: PHOX2B
• Identification du gène (NCBI): 8929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

Publications

Species	Application	Title
rat	WB	Stem Cells Transl Med; Neural Stem Cell-Derived Exosomal Netrin1 Contributes to Neuron Differentiation of Mesenchymal Stem Cells in Therapy of Spinal Bifida Aperta.; Authors - Ling Ma
mouse	IHC	Mol Metab; Ablation of intact hypothalamic and/or hindbrain TrkB signaling leads to perturbations in energy balance.; Authors - Ceren Ozek
human	IF	Sci Rep; Generating trunk neural crest from human pluripotent stem cells.; Authors - Miller Huang
human	IF	Sci Rep; Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating.; Authors - Yuzo Takayama
human	IHC	Int J Mol Sci; Deciphering the Molecular Mechanisms of Autonomic Nervous System Neuron Induction through Integrative Bioinformatics Analysis; Authors - Yuzo Takayama

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