Anticorps Monoclonal anti-PHOX2B

PHOX2B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F5F5

N° de cat : 66254-1-PBS

Synonymes

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B

---

Galerie de données de validation

• 

  WB analysis of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66254-1-Ig (PHOX2B Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of neuroblastoma using 66254-1-Ig (same clone as 66254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of neuroblastoma using 66254-1-Ig (same clone as 66254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 66254-1-Ig (same clone as 66254-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66254-1-PBS cible PHOX2B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PHOX2B Protéine recombinante Ag13196
• Nom complet: paired-like homeobox 2b
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 32-36 kDa
• Numéro d’acquisition GenBank: BC017199
• Symbole du gène: PHOX2B
• Identification du gène (NCBI): 8929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

{{ptg:RelatedPrimaryAntibodies}}