Anticorps Polyclonal de lapin anti-PHYH

PHYH Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12858-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human liver using 12858-1-AP (same clone as 12858-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 12858-1-AP (same clone as 12858-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 12858-1-AP (same clone as 12858-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12858-1-PBS cible PHYH dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHYH Protéine recombinante Ag3569
• Nom complet: phytanoyl-CoA 2-hydroxylase
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 36 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC029512
• Symbole du gène: PHYH
• Identification du gène (NCBI): 5264
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHYH (Phytanoyl-CoA 2-hydroxylase), also known as Phytanic acid oxidase or Phytanoyl-CoA alpha-hydroxylase, is a peroxisomal enzyme which converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. It catalyzes the first step in the alpha-oxidation of phytanic acid, a branched-chain fatty acid. Mutations in this gene can cause Refsum disease (RD) and deficient protein activity can cause Zellweger syndrome and rhizomelic chondrodysplasia punctata. This antibody (12858-1-AP) detects two bands: 36kd and 70kd. The 70kd band may probably be the dimeric form of PHYH.

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