Anticorps Polyclonal de lapin anti-PHYHIPL

PHYHIPL Polyclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 16937-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 16937-1-AP (same clone as 16937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16937-1-AP (PHYHIPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 16937-1-AP (same clone as 16937-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 16937-1-AP (PHYHIPL antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16937-1-PBS cible PHYHIPL dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHYHIPL Protéine recombinante Ag10435
• Nom complet: phytanoyl-CoA 2-hydroxylase interacting protein-like
• Masse moléculaire calculée: 376 aa, 43 kDa
• Poids moléculaire observé: 39 kDa and 42-45 kDa
• Numéro d’acquisition GenBank: BC011268
• Symbole du gène: PHYHIPL
• Identification du gène (NCBI): 84457
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PHYHIPL, a kind of mitochondrial protein, is associated with energy production, oxidative stress and organelle dynamics. It is reported that global ischemia is related to the change of PHYHIPL protein level. Compared with the control group, the level of APOE (may be involved in abnormal regulation of mitochondrial function) is significantly increased after ischemic stimulation, while the APOE genotype has a effect on PHYHIPL protein level. 16937-1-AP detects 39 kDa isoform and 42-45 kDa isoform in SDS-PAGE. (PMID: 21878944)

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