Anticorps Monoclonal anti-PI3 Kinase p110 Alpha

• Phare
• Validé par KD/KO

PI3 Kinase p110 Alpha Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1F6A7

N° de cat : 67071-1-PBS

Synonymes

PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha

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Galerie de données de validation

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  WB analysis of U2OS using 67071-1-Ig (same clone as 67071-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67071-1-Ig (PI3 Kinase p110 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67071-1-Ig (same clone as 67071-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67071-1-Ig (PIK3CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67071-1-Ig (same clone as 67071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67071-1-Ig (same clone as 67071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67071-1-Ig (same clone as 67071-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PI3 Kinase p110 Alpha antibody (67071-1-Ig, Clone: 1F6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 67071-1-Ig (same clone as 67071-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human PI3 Kinase p110 Alpha (67071-1-Ig, Clone:1F6A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67071-1-PBS cible PI3 Kinase p110 Alpha dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PI3 Kinase p110 Alpha Protéine recombinante Ag17392
• Nom complet: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Masse moléculaire calculée: 1068 aa, 124 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC113601
• Symbole du gène: PIK3CA
• Identification du gène (NCBI): 5290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

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