Anticorps Monoclonal anti-PI3 Kinase p85 Alpha

• Phare
• Validé par KD/KO

PI3 Kinase p85 Alpha Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4G3C11

N° de cat : 60225-1-PBS

Synonymes

P85a; PIK3R1, PI3K p85 alpha, PI3K p85 α, PIK3R1, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

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Galerie de données de validation

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  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60225-1-Ig (same clone as 60225-1-PBS)

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60225-1-Ig (same clone as 60225-1-PBS)

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 60225-1-Ig (same clone as 60225-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 60225-1-Ig (same clone as 60225-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60225-1-Ig (same clone as 60225-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60225-1-Ig (same clone as 60225-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 60225-1-Ig (same clone as 60225-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 60225-1-Ig (same clone as 60225-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 60225-1-Ig (same clone as 60225-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 60225-1-Ig (same clone as 60225-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 60225-1-Ig (same clone as 60225-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 60225-1-Ig (same clone as 60225-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

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  IP experiment of RAW 264.7 using 60225-1-Ig (same clone as 60225-1-PBS)

  IP result of anti-PI3 Kinase p85 Alpha (IP:60225-1-Ig, 5ug; Detection:60225-1-Ig 1:500) with RAW 264.7 cells lysate 1480ug. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60225-1-Ig (same clone as 60225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60225-1-PBS cible PI3 Kinase p85 Alpha dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PI3 Kinase p85 Alpha Protéine recombinante Ag2344
• Nom complet: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC030815
• Symbole du gène: PI3 Kinase p85 Alpha
• Identification du gène (NCBI): 5295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.

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