Anticorps Monoclonal anti-PI3 Kinase p85 Beta

PI3 Kinase p85 Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B3B6

N° de cat : 67644-1-PBS

Synonymes

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2

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Galerie de données de validation

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  WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PI3 Kinase p85 Beta antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PIK3R2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67644-1-Ig (same clone as 67644-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p85 Beta antibody (67644-1-Ig, Clone: 2B3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67644-1-PBS cible PI3 Kinase p85 Beta dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PI3 Kinase p85 Beta Protéine recombinante Ag14369
• Nom complet: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Masse moléculaire calculée: 728 aa, 82 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC014170
• Symbole du gène: PI3 Kinase p85 Beta
• Identification du gène (NCBI): 5296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

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